FITC标记的LIM结构域激酶2抗体
产品名称： FITC标记的LIM结构域激酶2抗体
英文名称： Anti-LIM kinase 2/FITC
产品编号： HZ-18255R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-LIM kinase 2/FITC Conjugated antibody
FITC标记的LIM结构域激酶2抗体
| 英文名称 | Anti-LIM kinase 2/FITC |
| 中文名称 | FITC标记的LIM结构域激酶2抗体 |
| 别 名 | LIM domain kinase 2; LIMK 2; LIMK-2; LIMK2; LIMK2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 72kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LIM kinase 2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Subcellular Location: Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. Tissue Specificity: Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. Post-translational modifications: Phosphorylated on serine and/or threonine residues by ROCK1. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 2 LIM zinc-binding domains. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain. Database links: Entrez Gene: 3985 Human Entrez Gene: 16886 Mouse Entrez Gene: 29524 Rat Omim: 601988 Human SwissProt: P53671 Human SwissProt: O54785 Mouse SwissProt: P53670 Rat Unigene: 474596 Human Unigene: 124176 Mouse Unigene: 390323 Mouse Unigene: 442189 Mouse Unigene: 11013 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
大约有40个已知的真核LIM蛋白，因此命名为它们包含的LIM结构域。LIM结构域是含有2个锌指的高度保守的富含半胱氨酸的结构。虽然锌指通常通过结合DNA或RNA发挥作用，但LIM基序可能介导蛋白质-蛋白质相互作用。LIM激酶-1和LIM激酶-2属于一个具有2个N-末端LIM基序和C-末端蛋白激酶结构域的独特组合的小亚家族。这个基因编码的蛋白质被ROCK磷酸化和激活，ROCK是Rho的下游效应子，而编码的蛋白质反过来磷酸化辅丝蛋白，抑制其肌动蛋白的解聚活性。据认为，这种途径有助于Rho诱导的肌动蛋白细胞骨架重组。已经发现至少三个编码不同亚型的转录变体。[ RefSeq，JUL 2008 ]