DIG DNA 标记试剂盒
 
DIG DNA Labeling Kit：Random primed DNA labeling with digoxigenin-dUTP, alkali-labile
 
用DIG-11-dUTP对DNA作随机引物扩增法标记。带DIG标记的探针可用于各种类型膜杂交，标记的探针通过酶联免疫法检测。
 
产品号
 产品规格
 厂商
 价格
 
1175033

11175033910
 Kit(40个标记反应)：(40 labeling reactions)
 Roche
 6545
 

DIG DNA Labeling Kit

Application

Random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. The standard reaction with 1 g template DNA will give a yield of 260 ng DIG-labeled DNA after 1-hour incubation at 37°C, or 780 ng after 20-hour incubation. DIG-labeled DNA probes are used in all types of nucleic-acid filter hybridizations. DIG-labeled nucleic-acid hybrids are detected by an enzyme-linked immunoassay using anti-DIG-AP conjugate and chromogenic or chemiluminescence AP substrates.

Product Description

Sensitivity and specificity: A single-copy human gene (tPA gene) is detected with a DIG-labeled probe in a Southern blot of 1 g digested human placenta DNA。

Contents

1.       Unlabeled Control DNA 1, 20 l, [100 g/ml] 

2.       Unlabeled Control DNA 2, 20 l, [200 g/ml] 

3.       DNA Dilution Buffer, 2 x 1 ml 

4.       DIG-labeled Control DNA, 50 l, [5.2 g/ml] 

5.       Hexanucleotide Mix, 10x, 80 l 

6.       dNTP Labeling Mixture, 10x, 80 l 

7.       Klenow Enzyme, labeling grade, 40 l, [2 U/l]