MYLK (N-term) Peptide
产品名称: MYLK (N-term) Peptide
英文名称: MYLK (N-term) Peptide
产品编号: P2960
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MYLK.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MYLK.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing, and polymorphic marker.
Watterson DM, Schavocky JP, Guo L, Weiss C, Chlenski A, Shirinsky VP, Van Eldik LJ, Haiech J.J Cell Biochem. 1999 Dec 1;75(3):481-91.
- 2.
- A single human myosin light chain kinase gene (MLCK; MYLK).
Lazar V, Garcia JG.Genomics. 1999 Apr 15;57(2):256-67.
- 3.
- Myosin light chain kinase in endothelium: molecular cloning and regulation.
Garcia JG, Lazar V, Gilbert-McClain LI, Gallagher PJ, Verin AD.Am J Respir Cell Mol Biol. 1997 May;16(5):489-94.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of MYLK polyclonal antibody (Cat # PAB3182) in mouse brain tissue lysate. MYLK (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody (Cat # PAB3182) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 4638
- Protein Accession#:
- Q9C0L5
- Gene Name:
- MYLK
- Gene Alias:
- DKFZp686I10125,FLJ12216,KRP,MLCK,MLCK1,MLCK108,MLCK210,MSTP083,MYLK1,smMLCK
- Gene Description:
- myosin light chain kinase
- Omim ID:
- 600922
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq
- Other Designations:
- OTTHUMP00000180642,OTTHUMP00000180643,myosin, light polypeptide kinase,smooth muscle myosin light chain kinase
- Interactome
- Related Disease
- Asthma
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- Coronary Artery Disease
- Critical Illness
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- Edema
- Genetic Predisposition to Disease
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- Kidney Failure, Chronic
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