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 R-spondin-1 / RSPO1 Antibody 
 
 
 
 
 
 
 
 
 
Order or Inquire for RSPO1 Antibody product
 
 
 
 
 
 
 
Detection limit is 0.00975 ng/well in ELISA
 
 
 
 
 
 
 
 
 
 
R-spondin-1 / RSPO1抗体产品信息
 
 
 
 
免疫原 : 
 
Recombinant human RSPO1 protein ( Catalog#11083-H08H )
 
 
 
Antibody Type : 
 
Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
 
 
 
 
克隆号 :
 
 
106
 
 
 
抗体宿主 :
 
 
Rabbit IgG
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human RSPO1 / R-spondin-1 ( rh RSPO1 ; Catalog#11083-H08H ; NP_001033722.1 ; Met 1 - Ala 263 ). 
 
 
 
 
R-spondin-1 / RSPO1 Antibody Usage Guide
 
 
 
 
 
特异性 : 
 
 
Human RSPO1 / R-spondin-1 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
Direct ELISA :
 
This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human RSPO1. The detection limit for human RSPO1 is approximately 0.00975 ng/well
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
 
R-spondin-1 / RSPO1 Antibody Related Products & Topics
 
 
R-spondin-1 / RSPO1 Antibody 相关领域:
 
Proteins:
 
Antibodies:
 
 
R-spondin-1 / RSPO1 抗体背景综述
 
R-Spondin 1 (Roof plate-specific Spondin 1), also known as RSPO1 and Cristin 3, is a secreted protein which belongs to the R-Spondin family. The R-Spondin (RSPO) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. R-Spondin1 regulates cellular responsiveness to Wnt ligands by modulating the cell-surface levels of the coreceptor LRP6. R-Spondin1 activity critically depends on the presence of canonical Wnt ligands and LRP6. R-Spondin1 does not directly activate LRP6, it interferes with DKK1/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 levels on the cell surface. Mature human R-Spondin1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. Human R-Spondin1 shares 89%, 87%, 92%, 91%, 91%, and 89% aa identity with mouse, rat, equine, canine, caprine and bovine R-Spondin1, respectively. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY. 
 
参考文献
 
 
 
Parma, P. et al., 2006, Nature genetics. 38 (11): 1304-9.
 
Capel, B., 2006, Nature genetics. 38 (11) :1233-4.
 
Binnerts, ME. et al., 2007, Proc Natl Acad Sci. 104 (37): 14700-5.
 
Wilhelm, D., 2007, Bioessays.  29 (4): 314-8.
 
Zhao, J. et al., 2009, Proc Natl Acad Sci. 106 (7) :2331-6.