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MHC Class I Polypeptide-Related Sequence B ( MICB ) Antibody Datasheet
 
 
 
MICB抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human MICB protein  ( Catalog#10759-H08H )
 
 
 
 
 
克隆号 :
 
 
 
9A11C3
 
 
 
 
抗体宿主 :
 
 
Mouse  IgG1
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human  MICB ( rh MICB ; Catalog#10759-H08H ; NP_005922.2 ; Met 1- Gly 298 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
MICB抗体使用指南
 
 
 
 
 
特异性 : 
 
 
 
Human MICB / MIC-B
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
Western blot ：
 
This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect MICB in WB. Using a DAB detection system, the detection limit for human MICB is approximately 1ng/lane under non-reducing conditions and 2ng/lane under reducing conditions.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.  Avoid repeated freeze-thaw cycles.
 
 
 
 
 MICB Antibody Related Products & Topics
 
 
相关研究领域:
 
Immunology>>Adaptive Immunity>>Major Histocompatibility Complex>>MICB
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
Size/Price
 
 
 
MICB
 
Human
 
MICB/Fc Protein, Recombinant 
 
10759-H03H
 
100µg($290) ; Order
 
 
 
MICB
 
Human
 
MICB Protein. Recombinant 
 
10759-H08H
 
100µg($290) ; Order
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
Size/Price
 
 
 
Human 
 MICB
 
WB
 
Mouse Monoclonal Antibody
 
10759-MM09
 
 200µg($95) 500µg($195)  Order
 
 
 
Human 
 MICB
 
ELISA
 
Mouse Monoclonal Antibody
 
10759-MM12
 
 200µg($95) 500µg($195)  Order
 
 
 
Human 
 MICB
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
10759-RP01
 
 500µg($150) 1mg($240)  Order
 
 
 
Human 
 MICB
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10759-RP02
 
100µg($250); Order
 
 
 
 
 
 MICB抗体背景综述
 
 
MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. Acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.
 
 
参考文献
 
 
 
Bauer, S. et al., 1999, Science. 285:727-729.
 
Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108.
 
Groh, V. et al., 2001, Nature Immunol. 2: 255-260.
 
Stephens, H., 2001, Trends Immunol. 22: 378-385.
 
Borrego, F. et al., 2002, Mol. Immunol. 38: 637-660.
 
Groh, V. et al., 2002, Nature. 419:734-738.
 
 
 
 
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