酪氨酸激酶衔接蛋白非催化区1(NCK1)重组蛋白

YB712Hu015
Recombinant Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1 (NCK1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 酪氨酸激酶衔接蛋白非催化区1(NCK1)重组蛋白[ PROPERTIES ]
Residues: Ala2~Ser377
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P16333
Host: E. coli
Subcellular Location: Cytoplasm, Endoplasmic
reticulum, Nucleus.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 酪氨酸激酶衔接蛋白非催化区1(NCK1)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 46.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
 酪氨酸激酶衔接蛋白非催化区1(NCK1)重组蛋白The sequence of the target protein is listed below.
AEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN
SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE
RE DE LS LI KG TKVIVME K CS DGWW R GSY NG QV GW FPS NYV TEE GDS P LG D
HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW
KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH
QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME
ELVEHYKKAP IFTSEQGEKL YLVKHLS