描述
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.
• Thermostability – Half life of 220 minutes at 50°C
• Yield – Reduced RNase H activity for more full-length cDNA
• Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
• Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3´ and 5´ RACE
Source: Purified
常用规格
| Optimal Reaction Temperature: |
50° C
|
| Final Product Size(s): |
12.3 kb or less
|
| Ribonuclease H Activity: |
Reduced
|
| Concentration: |
200 U⁄µl
|
| Reverse Transcriptase: |
SuperScript® III
|
| Form: |
Frozen
|
| Format: |
Tube(s)
|
| Template: |
ssRNA
|
| Sensitivity: |
Medium
|
| Product Size: |
10,000 units
|
| Enzyme Function: |
RNA dependent DNA Polymerase
|
| Shipping Condition: |
Dry Ice
|
| Number of Reactions: |
50 Reactions
|
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures.
|
内容和储存
SuperScript® III Reverse Transcriptase (10,000 units total, at 200U/µL) is supplied with a vial (1 mL) of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial (500 µL) of 100 mM DTT. Store at -20°C.
