RAD52 polyclonal antibody
产品名称： RAD52 polyclonal antibody
英文名称： RAD52 polyclonal antibody
产品编号： PAB10230
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RAD52.
- Immunogen:
- A synthetic peptide corresponding to amino acids 360-375 of human RAD52.
- Host:
- Rabbit
- Reactivity:
- Human
- Specificity:
- Reactivity occurs against human RAD52 protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells.
Park MS.J Biol Chem. 1995 Jun 30;270(26):15467-70.
- 2.
- The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues.
Shen Z, Denison K, Lobb R, Gatewood JM, Chen DJ.Genomics. 1995 Jan 1;25(1):199-206.
- 3.
- Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination.
Muris DF, Bezzubova O, Buerstedde JM, Vreeken K, Balajee AS, Osgood CJ, Troelstra C, Hoeijmakers JH, Ostermann K, Schmidt H, et al..Mutat Res. 1994 Nov;315(3):295-305.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis is shown using RAD52 polyclonal antibody (Cat # PAB10230) to detect human RAD52 present in a HeLa nuclear extract (panel B).
Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE.
Comparison to a molecular weight marker (not shown) indicates a band of ~ 63.0 KDa is detected.
Peptide competition (panel A) blocks the specific staining of this band.
The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 5,000 for 45 min.
- Entrez GeneID:
- 5893
- Protein Accession#:
- P43351;NP_002870
- Gene Name:
- RAD52
- Gene Alias:
- -
- Gene Description:
- RAD52 homolog (S. cerevisiae)
- Omim ID:
- 600392
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq
- Other Designations:
- DNA repair protein RAD52,RAD52 homolog,recombination protein RAD52,rhabdomyosarcoma antigen MU-RMS-40.23
- Gene Pathway
- Related Disease
- Adenocarcinoma
- Ataxia telangiectasia
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma
- Carcinoma, Papillary
- Colorectal Neoplasms
- DNA Damage
- Esophageal Neoplasms
- Genetic Predisposition to Disease
- Glioma
- Head and Neck Neoplasms
- Kidney Failure, Chronic
- Lung Neoplasms
- Meningeal Neoplasms
- Meningioma
- Multiple Sclerosis
- Neoplasms