Recombinant SARS-COV-2 Spike S1(Delta B.1.617.2) Protein, His Tag
产品名称： Recombinant SARS-COV-2 Spike S1(Delta B.1.617.2) Protein, His Tag
英文名称： Recombinant SARS-COV-2 Spike S1(Delta B.1.617.2) Protein, His Tag
产品编号： 94042ES25
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:09:52
使用范围： null
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产品描述
Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). First detected in India in December 2020, the B.1.617.2, or Delta variant, is considered a Variant of Concern (VOC) .
产品性质
|
别名 |
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
|
Accession |
QHD43416.1 |
|
表达区间及表达系统 |
Recombinant SARS-CoV-2 Spike S1 (Delta B.1.617.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R). |
|
分子量 |
Approximately 75.92 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
|
纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性 |
ELISA Data：Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. |
|
内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20 ℃保存，一年有效期。
复溶后，-20 TUNEology 波长可调检测卡盒-->