Synonyms:	EPLC-272HEPLC-272H（人肺癌鳞癌细胞）
Background:	established from a surgical lung specimen of a 57-year-old Caucasian man who underwent surgery for undifferentiated squamous cell carcinoma of the lung (without having received prior radiation or chemotherapy) in 1986
Species:	human (Homo sapiens)
Tissue:	lung
Disease:	epidermoid lung carcinoma
Gender:	N/A
Morphology:	adherent epithelial-like cells (without sharp contours) growing adherently as monolayers
Growth Mode:	N/A
Doubling Time:	ca. 60 hours
DNA Profile:	N/A Cell Line Authentication Service
Culture Medium:	RPMI-1640+20%FBS We strongly suggest to purchase the complete medium
Cryopreservation medium:	90%FBS+10%DMSO
Mycoplasma:	negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:	cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:	multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:	confirmed as human with IEF of AST, LDH, MDH
Cytogenetics:	human hypotriploid karyotype with 12% polyploidy - 66(59-70)<3n>XX, -Y, -4, +7, +8, -18, -19, -20, -21, +6mar, add(1)(p32), del(2)(q33), der(2;11)(q10;q10), add(3)(p25), i(5p), i(5q), add(6)(q11), add(7)(q33)x2-3, add(7)(p15), del(8)(p12-21), del(10)(p14)x1-2, add(11)(q24)x2, add(12)(p11-12), add(13)(q33), add(14)(p12), i(15q), der(17)t(3;17)(p11;p12), add(19)(q13), add(21)(p12), dmx2 - matches published karyotype
Virus	ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -