An integrated procedure for the measurement of Available 
Carbohydrates and Dietary Fiber in cereal products, fruit and 
vegetables and food products

Principle (Dietary Fiber):
     (α-amylase + amyloglucosidase)
(1) Starch + H₂O → glucose

                     (protease)
(2) Protein + H₂O → peptides

(3) Dietary fiber determined gravimetrically following 
      alcohol precipitation

Principle (Available Carbohydrates):
                        (sucrase / maltase + β-galactosidase)
(4) Sucrose, maltose and lactose → D-glucose + D-fructose 
                                                                    + D-galactose

                 (PGI, hexokinase + glucose-6-phosphate dehydrogenase)
(5) D-Glucose + D-fructose + ATP + NADP⁺ → gluconate-6-phosphate
                                                                        + NADPH + ADP + H⁺

Kit size:                             100 assays of each
Application examples: 
Food ingredients, food products and other materials
Method recognition:     
Dietary Fibre - AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
and AACC (Methods 32-05.01, 32-07.01 and 32-21.01)
 Advantages
Very cost effective 
All reagents stable for > 2 years after preparation 
High purity / standardised enzymes employed 
Only kit available 
Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
Simple format