Product Information

Transformation of plasmid DNA to competent E. Coli cells

1. Thaw competent cells on ice. 20–200µL per tube
2. Add max. 1-3µL of plasmid
3. Mix very gently!
4. Incubate the tubes on ice for 30 min
5. Heat shock the cells for 45 sec to 2 min at 42°C
6. Place the tubes immediately on ice for at least 2 min
7. Add 800µL of SOC medium to each tube
8. Incubate for 1 hour at 37°C and shake vigorously
9. Spin down briefly and remove most supernatants
10. Resuspend cell pellet with the rest SOC medium in the tube by pipetting
11. Plate out the suspension on a LB agar plate containing the appropriate antibiotic. Incubate the plates overnight at 37°C

Growth in Bacteria

·         Bacterial Resistance(s)

Ampicillin

·         Growth Temperature

37°C

·         Growth Strain(s)

DH5alpha

·         Copy number

High Copy

>sequence  5558bp

CTGCAGCCTGAATATGGGCCAAACAGGATATCTGTGGTAAGCAG

TTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGAACAGCTGAAT

ATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGC

TCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCCTCA

GCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGG

ACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTC

GCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATA

AAAGAGCCCACAACCCCTCACTCGGGGCGCCAGTCCTCCGATTG

ACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCA

GTTGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCC

TCTGAGTGATTGACTACCCGTCAGCGGGGGTCTTTCATTTGGGG

GCTCGTCCGGGATCGGGAGACCCCTGCCCAGGGACCACCGACCC

ACCACCGGGAGGTAAGCTGGCCAGCAACTTATCTGTGTCTGTCC

GATTGTCTAGTGTCTATGACTGATTTTATGCGCCTGCGTCGGTA

CTAGTTAGCTAACTAGCTCTGTATCTGGCGGACCCGTGGTGGAA

CTGACGAGTTCTGAACACCCGGCCGCAACCCTGGGAGACGTCCC

AGGGACTTTGGGGGCCGTTTTTGTGGCCCGACCTGAGGAAGGGA

GTCGATGTGGAATCCGACCCCGTCAGGATATGTGGTTCTGGTAG

GAGACGAGAACCTAAAACAGTTCCCGCCTCCGTCTGAATTTTTG

CTTTCGGTTTGGAACCGAAGCCGCGCGTCTTGTCTGCTGCAGCA

TCGTTCTGTGTTGTCTCTGTCTGACTGTGTTTCTGTATTTGTCT

GAAAATTAGGGCCAGACTGTTACCACTCCCTTAAGTTTGACCTT

AGATCACTGGAAAGATGTCGAGCGGCTCGCTCACAACCAGTCGG

TAGATGTCAAGAAGAGACGTTGGGTTACCTTCTGCTCTGCAGAA

TGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTAAC

CGAGACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGC

CCGCATGGACACCCAGACCAGGTCCCCTACATCGTGACCTGGGAA

GCCTTGGCTTTTGACCCCCCTCCCTGGGTCAAGCCCTTTGTACAC

CCTAAGCCTCCGCCTCCTCTTCTTCCATCCGCGCCGTCTCTCCCC

CTTGAACCTCCTCTTTCGACCCCGCCTCAATCCTCCCTTTATCCA

GCCCTCACTCCTTCTCTAGGCGCCGGCCGGATCCCAGTGTGGTGG

TACGTAGGAATTCGCCAGCACAGTGGTCGACCCTGTGGAATGTGT

GTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAA

GTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAA

AGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATC

TCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCC

CGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCT

GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCT

CTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAG

GCTTTTGCAAAAAGCTTACCATGACCGAGTACAAGCCCATGAAAA

AGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAA

AGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAG

AATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCC

TGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATG

TTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGC

TTGACATTGGGGAGTTCAGCGAGAGCCTGACCTATTGCATCTCCC

GCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAAC

TGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCG

CTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGAC

CGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCG

CGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACG

ACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGC

TTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGG

ATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAG

CGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACG

AGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGG

AGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAG

GATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACC

AACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTT

GGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGA

CTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGA

CCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCC

CCAGCACTCGTCCGAGGGCAAAGGAATATCGATAAAATAAAAGAT

TTTATTTAGTCTCCAGAAAAAGGGGGGAATGAAAGACCCCACCTG

TAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATG

GAAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTCAGG

AACAGATGGAACAGCTGAATATGGGCCAAACAGGATATCTGTGGT

AAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGAACAGC

TGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCC

CGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCC

TCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAA

GGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTT

CGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATA

AAAGAGCCCACAACCCCTCACTCGGGGCGCCAGTCCTCCGATTGA

CTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCAGT

TGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCT

GAGTGATTGACTACCCGTCAGCGGGGGTCTTTCATTTCCGACTTG

TGGTCTCGCTGCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACC

CGTCAGCGGGGGTCTTCACATGCAGCATGTATCAAAATTAATTTG

GTTTTTTTTCTTAAGTATTTACATTAAATGGCCATAGTTGCATTA

TUNEology 波长可调检测卡盒-->